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| HAL : hal-00023397, version 1 |
| Fiche détaillée |  Récupérer au format |
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| Applied and Environmental Microbiology 71 (2005) 6910-6917 |
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| An efficient procedure for purification of the obligate intracellular Wolbachia pipientis and representative amplification of its genome by multiple displacement amplification |
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| Patrick Mavingui 1Van Tran Van 1 |
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| (2005) |
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| procedure to representatively amplify Wolbachia genome by multiple displacement amplification from limited infected host tissue (0.2 g or 2 x 107 cell line). We obtained sufficient amounts (8 to 10 µg) of DNA of suitable quality for genomic studies with which we demonstrated that the amplified DNA contained all of the Wolbachia loci targeted. In addition, our data indicated that the genome of wRi strain, an obligatory endosymbiont of Drosophila simulans, shared similar overall architecture with its relative strain wMel. |
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| 1 : | Ecologie microbienne (EM) |
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CNRS : UMR5557 – INRA : UR1193 – Université Claude Bernard - Lyon I – Ecole Nationale Vétérinaire de Lyon |
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| 2 : | HELIX (INRIA Rhône-Alpes) |
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INRIA – CNRS : UMR5558 – Université Claude Bernard - Lyon I |
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| 4 |
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| Domaine | : | Sciences du Vivant/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique |
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| Wolbachia pipientis – wRi – genome amplification – multiple-displacement amplification |
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| hal-00023397, version 1 | |
| http://hal.archives-ouvertes.fr/hal-00023397 | |
| oai:hal.archives-ouvertes.fr:hal-00023397 | |
| Contributeur : Christine Delorme | |
| Soumis le : Lundi 13 Novembre 2006, 16:46:53 | |
| Dernière modification le : Vendredi 26 Janvier 2007, 10:24:48 | |
