Use of coherent control for selective two-photon fluorescence microscopy in live organisms.

Jennifer P. Ogilvie (1) , Delphine Débarre (2) , Xavier Solinas (2) , Jean-Louis Martin (2) , Emmanuel Beaurepaire (2) , Manuel Joffre (2)

1 Department of Physics/Biophysics Research Division
2 LOB - Laboratoire d'optique et biosciences

We demonstrate selective fluorescence We demonstrate selective fluorescence excitation of specific molecular species in live organisms by using coherent control of two-photon excitation. We have acquired quasi-simultaneous images in live fluorescently-labeled Drosophila embryos by rapid switching between appropriate pulse shapes. Linear combinations of these images demonstrate that a high degree of fluorophore selectivity is attainable through phase-shaping. Broadband phase-shaped excitation opens up new possibilities for single-laser, multiplex, in-vivo fluorescence microscopy. © 2006 Optical Society of America

Domaines: Optique [physics.optics]; Ingénierie biomédicale

Jennifer P. Ogilvie, Delphine Débarre, Xavier Solinas, Jean-Louis Martin, Emmanuel Beaurepaire, et al.. Use of coherent control for selective two-photon fluorescence microscopy in live organisms.. Optics Express, 2006, 14 (2), pp.759-766. ⟨10.1364/OPEX.14.000759⟩. ⟨hal-00018766⟩