Crystallographic Study of an Adenovirus-Induced Protein Crystal in KB cells: a structural model

Alain Lifchitz (1) , Alexandre Rimsky (1) , Gérard Torpier (2) , Pierre Boulanger (2)

1 LMCP - Laboratoire de minéralogie, cristallographie de Paris
2 Récepteurs nucléaires, lipoprotéines et athérosclérose

The physical parameters of a protein crystal induced by adenovirus type 5 in KB cells were determined from images of positively contrasted, sectioned intranuclear crystals. Fourier transforms of digitized images showed that the net appearing on transverse sections of the crystals, thus far considered as hexagonal, was in fact pseudohexagonal, rectangular. The three-dimensional characteristics of the crystal unit cell were: a0= 630, b0= 1200, c0=900 Å; alpha=gamma=90, beta=95.5°. The space group was P21. The asymmetric unit of the crystal was found to consist of a tubule centred by a densely stained tubular filament; its calculated molecular weight was 130 MDaltons. A three-dimensional model was reconstructed from the crystallographic data.

Mots clés: image processing; crystal structure

Domaines: Autre [q-bio.OT]; Biochimie [q-bio.BM]; Science des matériaux [cond-mat.mtrl-sci]

Alain Lifchitz, Alexandre Rimsky, Gérard Torpier, Pierre Boulanger. Crystallographic Study of an Adenovirus-Induced Protein Crystal in KB cells: a structural model. Acta Crystallographica Section B : Structural Science, Crystal Engineering and Materials [2014-..], 1975, 31, pp.441-445. ⟨10.1107/S0567740875002981⟩. ⟨hal-00015153⟩