Tetanus neurotoxin-mediated cleavage of cellubrevin impairs epithelial cell migration and integrin-dependent cell adhesion - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2005

Tetanus neurotoxin-mediated cleavage of cellubrevin impairs epithelial cell migration and integrin-dependent cell adhesion

Résumé

A role for endocytosis and exocytosis in cell migration has been proposed but not yet demonstrated. Here we show that cellubrevin, an early endosomal v-SNARE, mediates trafficking in the lamellipod of migrating epithelial cells and partially colocalizes with markers of focal contacts. Expression of tetanus neurotoxin, which selectively cleaves cellubrevin, significantly reduced the speed of migrating epithelial cells. Furthermore, expression of tetanus neurotoxin enhanced the adhesion of epithelial cells to collagen, laminin, fibronectin and E-cadherin, altered spreading on collagen, and impaired the recycling of ?1 integrins. These results suggest that cellubrevin-dependent membrane trafficking participates in cell motility through the regulation of cell adhesion.
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Dates et versions

hal-00007763 , version 1 (02-08-2005)

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Véronique Proux-Gillardeaux, Julie Gavard, Theano Irinopoulou, René-Marc Mège, Thierry Galli. Tetanus neurotoxin-mediated cleavage of cellubrevin impairs epithelial cell migration and integrin-dependent cell adhesion. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102, pp.6362-6367. ⟨10.1073/pnas.0409613102⟩. ⟨hal-00007763⟩
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