Figure 7. RT-PCR analysis of OxR expression in epithelial and non-epithelial fractions of rat jejunal mucosa
OX1R (top panel), OX2R (middle panel) amplicon from CHO/OX1R cells and CHO/OX2R (positive controls) are shown in lane 1 and 3, respectively. Expression of OX1R transcripts was found only in epithelial cells fractions (lane 5), whereas expression of OX2R transcripts was found mainly in non-epithelial cells (lane 7) with a small positive signal in epithelial cell fraction (lane 5) which may be ascribed to the presence of enteroendocrine cells in the epithelial fraction. Bottom panel : expression of GADPH transcripts. Lane 2, 4, 6, 8 : negative controls of RT. Lane 9 : negative control of PCR.