Figure 4.
RT-PCR (A) and western blot (B) analysis of TGFβ1 in Ctrl, P2r0 and P3r0 rats and after refeeding (P2r2, P2r6, P2r24, P3r2, P3r6, P3r24). Mean +/− SEM (n=5 per group). (C) TGFβ1 immunohistochemical localization in Ctrl, P2r0, P3r0, P2r2 and P3r2 rats. TGFβ1 is localized in the cytoplasm of intestinal epithelial cells and is mainly observed in the intestinal villi of control and refed animals (6h and 24h refeeding data not shown).