Genelength as a regulator for ribosome recruitmentand protein synthesis: theoretical insights
Résumé
rotein synthesis rates are determined, at the translational level, by properties of the transcript’s sequence. The e ciency of an mRNA can be tuned by varying the ribosome binding sites controlling the recruitment of the ribosomes, or the codon usage establishing the speed of protein elongation. In this work we propose transcript length as a further key determinant of translation e ciency. Based on a physical model that considers the kinetics of ribosomes advancing on the mRNA and di using in its surrounding, as well as mRNA circularisation and ribosome drop-o , we explain how the transcript length may play a central role in establishing ribosome recruitment and the overall translation rate of an mRNA. According to our results, the proximity of the 3′ end to the ribosomal recruitment site of the mRNA could induce a feedback in the translation process that would favour the recycling of ribosomes. We also demonstrate how this process may be involved in shaping the experimental ribosome density-gene length dependence. Finally, we argue that cells could exploit this mechanism to adjust and balance the usage of its ribosomal resources.